Description
Principle of the assay: mouse CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL16 has been precoated onto 96-well plates. Standards(E.coli,N27-P114) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL16 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2.1 These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surfacebound molecule, as well as a soluble chemokine.2 CXCL16 is produced by dendritic cells found in the T cell zonesof lymphoid organs, and by cells found in the red pulp of the spleen.1 Cells that bind and migrate in response toCXCL16 include several subsets of T cells, and natural killer T (NKT) cells.1 CXCL16 interacts with the chemokinereceptor CXCR6, also known as Bonzo.1, 3 Expression of CXCL16 is induced by the inflammatory cytokinesIFN-gamma and TNF-alpha.2 The gene for human CXCL16 is located on chromosome 17p13.4 The standardproduct used in this kit is recombinant mouse CXCL16, consisting of 88 amino acids with the molecular mass of 9.9KDa